Itga4^tm1Mgin
Targeted Allele Detail

Summary

• Symbol: Itga4^tm1Mgin
• Name: integrin alpha 4; targeted mutation 1, Mark H Ginsberg
• MGI ID: MGI:3620620
• Synonyms: alpha4(Y991A)
• Gene: Itga4 Location: Chr2:79085770-79163467 bp, + strand Genetic Position: Chr2, 47.38 cM
• Alliance: Itga4^tm1Mgin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106471
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A point mutation was introduced into the alpha4 integrin tail to inhibit paxillin protein binding with little detectable effect on the binding of other proteins. The Y991A mutation was engineered into exon 28. A floxed TK/neomycin slection cassette was inserted upstream of exon 28 and and additional loxP site was inserted downstream. Cre recombinase was used to remove selectable cassettes from the vector leaving the mutated exon 28 floxed. (J:106471)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itga4 Mutation: 62 strains or lines available

References

• Original: J:106471 Feral CC, et al., Blocking the alpha4 integrin-paxillin interaction selectively impairs mononuclear leukocyte recruitment to an inflammatory site. J Clin Invest. 2006 Mar;116(3):715-23
• All: 11 reference(s)