Rap1gds1^tm1Ytk
Targeted Allele Detail

Summary

• Symbol: Rap1gds1^tm1Ytk
• Name: RAP1, GTP-GDP dissociation stimulator 1; targeted mutation 1, Yoshimi Takai
• MGI ID: MGI:3613572
• Synonyms: Smg GDS-, SmgGDS^-
• Gene: Rap1gds1 Location: Chr3:138631663-138780962 bp, - strand Genetic Position: Chr3, 64.52 cM
• Alliance: Rap1gds1^tm1Ytk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62244
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 1.7 kb of genomic sequence including the 3' half of exon 5, and exons 6 and 7, removing the portion of the gene that encodes amino acids 212-343. Western blot analysis using a carboxy terminal specific antibody did not detect protein in brain tissue from homozygous mutant mice. RT-PCR analysis of brain did not detect expression in homozygous mutant mice. (J:62244)

Expression

In Structures Affected by this Mutation:

16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rap1gds1 Mutation: 69 strains or lines available

References

• Original: J:62244 Takakura A, et al., Involvement of a small GTP-binding protein (G protein) regulator, small G protein GDP dissociation stimulator, in antiapoptotic cell survival signaling. Mol Biol Cell. 2000 May;11(5):1875-86
• All: 4 reference(s)