Pdpk1^tm1Bcol
Targeted Allele Detail

Summary

• Symbol: Pdpk1^tm1Bcol
• Name: 3-phosphoinositide dependent protein kinase 1; targeted mutation 1, Barry J Collins
• MGI ID: MGI:3608995
• Synonyms: PDK1^131M
• Gene: Pdpk1 Location: Chr17:24292654-24369898 bp, - strand Genetic Position: Chr17, 12.23 cM, cytoband A3.3
• Alliance: Pdpk1^tm1Bcol page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102388
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A targeting construct was designed to replace arginine with methionine at position 131 (R131M) in exon 4, disrupting the phopshate groove but retaining catalytic activity and integrity of the hydrophobic groove. Protein was expressed normally in mutant ES cells. (J:102388)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdpk1 Mutation: 138 strains or lines available

References

• Original: J:102388 Collins BJ, et al., In vivo role of the phosphate groove of PDK1 defined by knockin mutation. J Cell Sci. 2005 Nov 1;118(Pt 21):5023-34
• All: 1 reference(s)