Pik3r1^tm1Lca
Targeted Allele Detail

Summary

• Symbol: Pik3r1^tm1Lca
• Name: phosphoinositide-3-kinase regulatory subunit 1; targeted mutation 1, Lewis C Cantley
• MGI ID: MGI:3607981
• Synonyms: P85alphaloxP, Pik3r1^flox, pik3r1^loxP
• Gene: Pik3r1 Location: Chr13:101817269-101904725 bp, - strand Genetic Position: Chr13, 53.92 cM
• Alliance: Pik3r1^tm1Lca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102176
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed such that exon 7 is flanked by loxP sequences. Upon cre-mediated deletion of the exon, splicing of upstream exons 6, 1b or 1c directly into exon 8 results in a frameshift mutation producing an immediate stop codon. As a result the p85alpha isoform is truncated and p55alpha and p50alpha are essentially abolished. Flp mediated recombination removed the neo cassette. Western blot demonstrates the floxed allele is expressed just like the wild-type. (J:102176)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pik3r1^tm1Lca

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3r1 Mutation: 55 strains or lines available

References

• Original: J:102176 Luo J, et al., Class IA phosphoinositide 3-kinase regulates heart size and physiological cardiac hypertrophy. Mol Cell Biol. 2005 Nov;25(21):9491-502
• All: 42 reference(s)