Gria4^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Gria4^tm1Dgen
• Name: glutamate receptor, ionotropic, AMPA4 (alpha 4); targeted mutation 1, Deltagen
• MGI ID: MGI:3604485
• Synonyms: Gria4^KO
• Gene: Gria4 Location: Chr9:4417896-4796234 bp, - strand Genetic Position: Chr9, 2.46 cM
• Alliance: Gria4^tm1Dgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101679
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Genotyping (long range PCR and genomic sequencing) for this allele revealed that the 5' half of intron 3-lacZ-neo-intron 3 construct inserted into Gria4 intron 3 resulting in truncation of most of the lacZ gene and some of intron 3, instead of the intended homologous replacement of Gria4 exon 3 with a lacZ-neo cassette. No Gria4 RNA was detected in the brain by RT-PCR.

Expression

In Mice Carrying this Mutation:

32 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gria4 Mutation: 72 strains or lines available

Notes

See also, data as provided by Deltagen, Inc.

References

• Original: J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 8 reference(s)