Tcra-J^tm1Slek
Targeted Allele Detail

Summary

• Symbol: Tcra-J^tm1Slek
• Name: T cell receptor alpha, joining region; targeted mutation 1, Barry P Sleckman
• MGI ID: MGI:3604459
• Synonyms: TCRalpha^sJ
• Gene: Tcra-J Location: unknown Genetic Position: Chr14, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101398
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Using ES cells with a single loxP site 5' of the endogenous TEA promoter, a targeting vector was used to insert a floxed neo, TEA promoter, TEA exon, Jalpha61 and Jalpha56 sequences downstream of the Jalpha region. Resultant ES cells were transfected with cre recombinase, leading to the 66-kb deletion between the loxP sites 5' of the TAE endogenous promoter and 3' of the neomycin resistant gene. Only the Jalpha56 could give rise to a functional Jalpha arrangement in mutant mice. These mice have increased thymocyytes but reduced mature alphabeta T cell numbers. (J:101398)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra-J Mutation: 0 strains or lines available

References

• Original: J:101398 Huang CY, et al., Revision of T cell receptor {alpha} chain genes is required for normal T lymphocyte development. Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14356-61
• All: 2 reference(s)