Ep300^tm1Pkb
Targeted Allele Detail

Summary

• Symbol: Ep300^tm1Pkb
• Name: E1A binding protein p300; targeted mutation 1, Paul K Brindle
• MGI ID: MGI:3578128
• Synonyms: p300^KIX
• Gene: Ep300 Location: Chr15:81470329-81536278 bp, + strand Genetic Position: Chr15, 38.16 cM
• Alliance: Ep300^tm1Pkb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98329
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A floxed neomycin selection cassette was inserted upstream of the exon encoding the KIX domain and the KIX containing exon itself was mutated so that the tyrosine at amino acid residue 630 became alanine, the alanine at residue 634 became glutamine and the tyrosine at residue 638 became alanine (Y630A, A634Q and Y638A). Transient cre expression then excised the selection cassette. Expression and stability of the mutated product were normal. (J:98329)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ep300 Mutation: 97 strains or lines available

References

• Original: J:98329 Kasper LH, et al., A transcription-factor-binding surface of coactivator p300 is required for haematopoiesis. Nature. 2002 Oct 17;419(6908):738-43
• All: 3 reference(s)