Grid2tm1(cre/PGR*)Ksak
Targeted Allele Detail
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Symbol: |
Grid2tm1(cre/PGR*)Ksak |
Name: |
glutamate receptor, ionotropic, delta 2; targeted mutation 1, Kenji Sakimura |
MGI ID: |
MGI:3577356 |
Synonyms: |
D2CPR, GluRdelta2CrePR, Grid2tm1(cre/PGR)Ksak |
Gene: |
Grid2 Location: Chr6:63232860-64681307 bp, + strand Genetic Position: Chr6, 29.77 cM
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Alliance: |
Grid2tm1(cre/PGR*)Ksak page
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Germline Transmission: |
Earliest citation of germline transmission:
J:97754
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Parent Cell Line: |
Bruce 4 (ES Cell)
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Strain of Origin: |
B6.Cg-Thy1a
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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mifepristone |
Mutations: |
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Insertion, Intragenic deletion
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Grid2tm1(cre/PGR*)Ksak expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Grid2 (MGI:95813) |
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Mutation details: The targeting vector consisting of a fusion protein of Cre recombinase plus a modified ligand binding domain of the human progesterone receptor and pgk-neo-polyA cassette was inserted in the translational initiation site of the Grid2 gene in frame. Approximately 900bp nucleotides from the initiation site of the endogenous sequence were deleted. The ligand-binding domain of human progesterone receptor was modified such that it binds and can be activated by the synthetic steroid, RU486, but not by the endogenous ligand, progesterone. Upon induction of inserted cre/PGR expression with RU486, activity was noted in mature Purkinje cells of the cerebellum.
(J:97754)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Grid2
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Grid2 Mutation: |
85 strains or lines available
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ES cell line = Bruce (no line given).
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Original: |
J:97754 Kitayama K, et al., Purkinje cell-specific and inducible gene recombination system generated from C57BL/6 mouse ES cells. Biochem Biophys Res Commun. 2001 Mar;281(5):1134-40 |
All: |
7 reference(s) |
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