Adamts5^tm1.1Eam
Targeted Allele Detail

Summary

• Symbol: Adamts5^tm1.1Eam
• Name: ADAM metallopeptidase with thrombospondin type 1 motif 5; targeted mutation 1.1, Elisabeth A Morris
• MGI ID: MGI:3576786
• Synonyms: ADAMTS5^-
• Gene: Adamts5 Location: Chr16:85655045-85698013 bp, - strand Genetic Position: Chr16, 48.34 cM
• Alliance: Adamts5^tm1.1Eam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97590
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 3 was flanked with loxP sites to allow disruption of the zinc-binding site and deletion of the conserved "Met-turn." Cre mediated recombination of the flanking loxP sites resulted in the deletion of exon 3 from Adamts5^tm1Eam. RT-PCR confirmed absence of exon 3 in mutant spleen and cartilage transcripts. A protein product from the transcript was not found in mutant cartilage. (J:97590)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adamts5 Mutation: 54 strains or lines available

References

• Original: J:97590 Glasson SS, et al., Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature. 2005 Mar 31;434(7033):644-8
• All: 4 reference(s)