Nherf2^{Gt(OST2298)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Nherf2^{Gt(OST2298)Lex}
• Name: NHERF family PDZ scaffold protein 2; gene trap OST2298, Lexicon Genetics
• MGI ID: MGI:3530645
• Synonyms: N2^-, NHERF-2^-, NHERF2^-, Slc9a3r2^{Gt(IRESBetageo)524Lex}
• Gene: Nherf2 Location: Chr17:24858255-24869279 bp, - strand Genetic Position: Chr17, 12.43 cM, cytoband A3.3
• Alliance: Nherf2^{Gt(OST2298)Lex} page

Mutation origin

• Mutant Cell Line: OST2298
• Germline Transmission: Earliest citation of germline transmission: J:128912
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene trap vector VICTR22 was inserted into intron 2. Western blot analysis revealed a complete absence of protein in various homozygous mutant tissues, including kidney, lung, stomach, heart, brain, and colon. (J:128912)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST2298	CG474352 ; (MGI Seq Detail)	3' RACE	Chr17:24860682-24861319 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  9 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nherf2 Mutation: 21 strains or lines available

References

• Original: J:128912 Broere N, et al., Cystic Fibrosis Transmembrane Conductance Regulator Activation Is Reduced in the Small Intestine of Na+/H+ Exchanger 3 Regulatory Factor 1 (NHERF-1)- but Not NHERF-2-deficient Mice. J Biol Chem. 2007 Dec 28;282(52):37575-84
• All: 15 reference(s)