Card11^tm1Vmd
Targeted Allele Detail

Summary

• Symbol: Card11^tm1Vmd
• Name: caspase recruitment domain family, member 11; targeted mutation 1, Vishva M Dixit
• MGI ID: MGI:3528226
• Synonyms: deltaCARD
• Gene: Card11 Location: Chr5:140858745-140986337 bp, - strand Genetic Position: Chr5, 79.42 cM
• Alliance: Card11^tm1Vmd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95800
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3, encoding the caspase activation and recruitment domain (CARD) were replaced with a lacZ reporter gene and a PGK-neo. Retention of stop codons downstream of the exon 2 splice acceptor site and a consensus Kozak start site for lacZ, as well as the splicing of exon 1 to exon 4 allowed an ORF that included lacZ. Immunoprecipitation and Western blot of mutant spleen cells confirmed production of a protein without the CARD. Beta-galactosidase activity was detected in thymus, spleen and lymph nodes, but negligible in bone marrow of heterozygous mice. (J:95800)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Card11 Mutation: 58 strains or lines available

Notes

ES cell line = GS (129S6/SvEvTac).

References

• Original: J:95800 Newton K, et al., Mice lacking the CARD of CARMA1 exhibit defective B lymphocyte development and impaired proliferation of their B and T lymphocytes. Curr Biol. 2003 Jul 15;13(14):1247-51
• All: 1 reference(s)