Grem1^tm1Rmh
Targeted Allele Detail

Summary

• Symbol: Grem1^tm1Rmh
• Name: gremlin 1, DAN family BMP antagonist; targeted mutation 1, Richard M Harland
• MGI ID: MGI:3054923
• Synonyms: Grem1^-, gremlin^LacZ, ld^grm
• Gene: Grem1 Location: Chr2:113579020-113588993 bp, - strand Genetic Position: Chr2, 57.43 cM
• Alliance: Grem1^tm1Rmh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84077
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The open reading frame, which lies on a single exon, was replaced with an in frame fusion of lacZ with the sequence encoding the first five residues followed by an FRT site flanked neomycin resistance gene cassette. Mice with and without the neo cassette have the same phenotype. LacZ expression in heterozygous mutant mice recapitulates that of wild-type mice. (J:84077)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grem1 Mutation: 16 strains or lines available

Notes

This mutation was determined through complementation analysis to be allelic to Grem1^ld-J (J:84077). Heterozygous mutant mice were viable and fertile. Serial skeletal stains of cartilage and bone of heterozygous mutant embryos and newborn mice showed no abnormalities in limb development.

References

• Original: J:84077 Khokha MK, et al., Gremlin is the BMP antagonist required for maintenance of Shh and Fgf signals during limb patterning. Nat Genet. 2003 Jul;34(3):303-7
• All: 12 reference(s)