Ciita^tm2Wrth
Targeted Allele Detail

Summary

• Symbol: Ciita^tm2Wrth
• Name: class II transactivator; targeted mutation 2, Walter Reith
• MGI ID: MGI:3052223
• Synonyms: C2ta p(III+IV)^-, Ciita^III-IV-, CIITA p(III+IV)^-, PIII+IV^-
• Gene: Ciita Location: Chr16:10297923-10346282 bp, + strand Genetic Position: Chr16, 5.65 cM, cytoband B1
• Alliance: Ciita^tm2Wrth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92170
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted 560 bp upstream of the ATG for the pIII transcript and another loxP site was inserted 90 bp downstream of the first exon encoding the pIV transcript, followed by an FRT-flanked neo. Transient FLP recombinase expression excised the neo and mice generated were crossed with CMV-Cre mice to excise the floxed pIII and pIV sequence. RT-PCR indicated transcripts of both were undetectable in mutant tissues. Gene expression from pI was unaffected. (J:92170)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ciita Mutation: 45 strains or lines available

References

• Original: J:92170 LeibundGut-Landmann S, et al., MHC class II expression is differentially regulated in plasmacytoid and conventional dendritic cells. Nat Immunol. 2004 Sep;5(9):899-908
• All: 11 reference(s)