Dnmt3b^tm5.1Enl
Targeted Allele Detail

Summary

• Symbol: Dnmt3b^tm5.1Enl
• Name: DNA methyltransferase 3B; targeted mutation 5.1, En Li
• MGI ID: MGI:3047833
• Synonyms: Dnmt3b^2lox, Dnmt3b^2loxP, Dnmt3b^fl, Dnmt3b^flox
• Gene: Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
• Alliance: Dnmt3b^tm5.1Enl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91108
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked PGK-neo cassette was inserted in the intron upstream of exon 19 and a third loxP site in the intron between exons 19 and 20. Cre-mediated recombination deleted the selection cassette in the germline, leaving loxP sites upstream and downstream of exon 19. When mice bearing the present mutation are crossed to Cre recombinase expressing mice, deletion of the floxed DNA segment leads to out-of-frame splicing of the mRNAs encoding all active isoforms of the protein in the tissues where Cre is expressed. (J:91108)

Expression

In Mice Carrying this Mutation:

14 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3b Mutation: 50 strains or lines available

References

• Original: J:91108 Kaneda M, et al., Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature. 2004 Jun 24;429(6994):900-3
• All: 43 reference(s)