Gabra6tm2(cre)Wwis
Targeted Allele Detail
|
Symbol: |
Gabra6tm2(cre)Wwis |
Name: |
gamma-aminobutyric acid type A receptor subunit alpha 6; targeted mutation 2, W Wisden |
MGI ID: |
MGI:3047798 |
Synonyms: |
delta alpha6Cre |
Gene: |
Gabra6 Location: Chr11:42197264-42211899 bp, - strand Genetic Position: Chr11, 25.03 cM
|
Alliance: |
Gabra6tm2(cre)Wwis page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:91624
|
Parent Cell Line: |
E14.1 (ES Cell)
|
Strain of Origin: |
129P2/OlaHsd
|
|
Allele Type: |
|
Targeted (Recombinase) |
Mutation: |
|
Insertion
|
|
|
Gabra6tm2(cre)Wwis expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Gabra6 (MGI:95618) |
|
|
|
|
Mutation details: Exon 8 was disrupted by insertion, via homologous recombination, of a TAG3 IRES-nlsCre-neo cassette, which contains stop codons in all reading frames followed by an encephalomyelocarditis virus internal ribosome entry site (IRES), the coding sequence for Cre recombinase flanked by the nuclear localization sequence and polyadenylation signal from SV40, and a neomycin resistance gene driven by the HSV promoter. Cre was shown, both by immunohistochemistry and by X-gal staining of brains of adult F1 progeny from a cross to a beta-galactosidase expressing Cre reporter strain, to be expressed uniformly in nuclei of all cells of the granule cell layer and, additionally, in a few Purkinje cells and in some small cells in the molecular layer.
(J:91624)
|
|
|
Activity: |
Tissue activity of this recombinase allele
|
Driver:
|
Gabra6
(mouse)
|
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
Original: |
J:91624 Aller MI, et al., Cerebellar granule cell Cre recombinase expression. Genesis. 2003 Jun;36(2):97-103 |
All: |
22 reference(s) |
|