Rps12-ps1/Xist^tm1.1Nbd
Targeted Allele Detail

Summary

• Symbol: Rps12-ps1/Xist^tm1.1Nbd
• Name: inactive X specific transcripts; targeted mutation 1, Neil Brockdorff
• MGI ID: MGI:3047793
• Synonyms: DeltaCJdeltaneo
• Gene: Xist Location: ChrX:102503979-102526839 bp, - strand Genetic Position: ChrX, 46.15 cM, cytoband D
• Alliance: Rps12-ps1/Xist^tm1.1Nbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85305
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: Transient cre expression excised the floxed neo in Rps12-ps1/Xist^tm1Nbd, which had a 2.5 kb sequence deletion between 5.5 and 8 kb upstream of Xist, including Rps12-ps1 and spanning a site thought to include an upstream promoter for Xist. RT-PCR showed presence of antisense transcription upstream of Xist in mutants where none is present in wild-type. (J:85305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xist Mutation: 8 strains or lines available

References

• Original: J:85305 Nesterova TB, et al., Skewing X chromosome choice by modulating sense transcription across the Xist locus. Genes Dev. 2003 Sep 1;17(17):2177-90
• All: 1 reference(s)