Xist^tm2Nbd
Targeted Allele Detail

Summary

• Symbol: Xist^tm2Nbd
• Name: inactive X specific transcripts; targeted mutation 2, Neil Brockdorff
• MGI ID: MGI:3047784
• Synonyms: Delta5'+neo
• Gene: Xist Location: ChrX:102503979-102526839 bp, - strand Genetic Position: ChrX, 46.15 cM, cytoband D
• Alliance: Xist^tm2Nbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85305
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A sequence between 1 and 9 kb upstream of the P1 start site was deleted and replaced with a floxed PGK-neomycin resistance gene. Southern blot and PCR confirmed recombination. RT-PCR of mutants indicated antisense expression was not affected and ectopic sense transcription was detected upstream of Xist, as well as elevated transcription within the Xist locus. (J:85305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xist Mutation: 8 strains or lines available

References

• Original: J:85305 Nesterova TB, et al., Skewing X chromosome choice by modulating sense transcription across the Xist locus. Genes Dev. 2003 Sep 1;17(17):2177-90
• All: 1 reference(s)