Rho^{tm2(RHO/GFP)Jhw}
Targeted Allele Detail

Summary

• Symbol: Rho^{tm2(RHO/GFP)Jhw}
• Name: rhodopsin; targeted mutation 2, John H Wilson
• MGI ID: MGI:3046186
• Synonyms: hrhoG, RGFP, Rho-eGFP
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^{tm2(RHO/GFP)Jhw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91038
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Rho^{tm2(RHO/GFP)Jhw} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RHO (6010)

  Rho	(MGI:97914)

  Expresses a human RHO gene fused to GFP

• Mutation details: The endogenous gene was first replaced with an HPRT minigene and then the HPRT minigene was replaced with the human RHO gene fused to GFP via homologous recombination. Western blot analysis indicated that expression of the human gene was about 80% that of the endogenous gene. GFP expression is seen in the eyes. (J:91038)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 48 strains or lines available

References

• Original: J:91038 Chan F, et al., Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):9109-14
• All: 14 reference(s)