Aldh1a3^tm1Pcn
Targeted Allele Detail

Summary

• Symbol: Aldh1a3^tm1Pcn
• Name: aldehyde dehydrogenase family 1, subfamily A3; targeted mutation 1, Pierre Chambon
• MGI ID: MGI:3043945
• Synonyms: Aldh1a3^L2
• Gene: Aldh1a3 Location: Chr7:66040640-66077225 bp, - strand Genetic Position: Chr7, 35.73 cM, cytoband C
• Alliance: Aldh1a3^tm1Pcn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90261
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neomycin cassette was inserted in the antisense orientation downstream of exon 9 while a third loxP site was inserted upstream of exon 8. Subsequently, the neomycin resistance gene was excised by transient transfection of properly target ES cells with a cre-expressing plasmid and leaving exons 8 and 9 which code for the catalytic site flanked by loxP sites. (J:90261)

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aldh1a3 Mutation: 25 strains or lines available

References

• Original: J:90261 Dupe V, et al., A newborn lethal defect due to inactivation of retinaldehyde dehydrogenase type 3 is prevented by maternal retinoic acid treatment. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14036-41
• All: 11 reference(s)