Ebf3^tm1Reed
Targeted Allele Detail

Summary

• Symbol: Ebf3^tm1Reed
• Name: early B cell factor 3; targeted mutation 1, Randall R Reed
• MGI ID: MGI:3037961
• Synonyms: O/E2^lacz
• Gene: Ebf3 Location: Chr7:136795402-136916174 bp, - strand Genetic Position: Chr7, 82.26 cM, cytoband F3-F4
• Alliance: Ebf3^tm1Reed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88585
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing a tau-lacZpA reporter with a floxed tk-neo component was inserted in the place of exons 1-6 by homologous recombination, and remained under transcriptional control of the endogenous promotor. The exons encoded the first 185 amino acids, which contain domains crucial for DNA binding and transactivation of the protein binding site. Subsequent cre-mediated recombination removed the tk-neo cassette. In situ hybridization indicated lack of endogenous gene expression in homozygous mutants. The expression pattern of lacZ recapitulated that of the endogenous gene. (J:88585)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ebf3 Mutation: 34 strains or lines available

References

• Original: J:88585 Wang SS, et al., Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection. Development. 2004 Mar;131(6):1377-88
• All: 1 reference(s)