Efnb3^tm1.2Henk
Targeted Allele Detail

Summary

• Symbol: Efnb3^tm1.2Henk
• Name: ephrin B3; targeted mutation 1.2, Mark Henkemeyer
• MGI ID: MGI:3026703
• Synonyms: ephrin-B3^T
• Gene: Efnb3 Location: Chr11:69444918-69451031 bp, - strand Genetic Position: Chr11, 42.8 cM
• Alliance: Efnb3^tm1.2Henk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87497
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The loxP-flanked lacZ cassette was removed from Efnb2^tm1.1Henk (frt-flanked neo excised from Efnb2^tm1Henk) via cre-mediated recombination, generating a stop codon in exon 5. The nonsense mutation precludes the translation of the cytoplasmic tail (beyond codon 264). This allele is expected to encode a protein similar to Efnb2^tm1.1Henk, but lacking beta-gal. Similarly, this mutation is expected to ablate reverse signaling while leaving forward signaling intact. (J:87497)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efnb3 Mutation: 11 strains or lines available

References

• Original: J:87497 Yokoyama N, et al., Forward signaling mediated by ephrin-B3 prevents contralateral corticospinal axons from recrossing the spinal cord midline. Neuron. 2001 Jan;29(1):85-97
• All: 6 reference(s)