Pmaip1^tm1Ast
Targeted Allele Detail

Summary

• Symbol: Pmaip1^tm1Ast
• Name: phorbol-12-myristate-13-acetate-induced protein 1; targeted mutation 1, Andreas Strasser
• MGI ID: MGI:2681649
• Synonyms: noxa^-
• Gene: Pmaip1 Location: Chr18:66591675-66598629 bp, + strand Genetic Position: Chr18, 39.52 cM, cytoband E1
• Alliance: Pmaip1^tm1Ast page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86466
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 which contain the two BH3 interaction domains were replaced with a floxed PGK-hygromycin resistance cassette. The resistance gene was excised in vivo by crossing to C57BL/6 Deleter mice. Gene disruption was confirmed by RT-PCR and by Northern blot analysis. (J:86466)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pmaip1 Mutation: 11 strains or lines available

References

• Original: J:86466 Villunger A, et al., p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science. 2003 Nov 7;302(5647):1036-8
• All: 39 reference(s)