Ccne2^tm1Boa
Targeted Allele Detail

Summary

• Symbol: Ccne2^tm1Boa
• Name: cyclin E2; targeted mutation 1, Bruno Amati
• MGI ID: MGI:2677700
• Synonyms: E2^-
• Gene: Ccne2 Location: Chr4:11191351-11204779 bp, + strand Genetic Position: Chr4, 5.07 cM, cytoband A2
• Alliance: Ccne2^tm1Boa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85497
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 12.3 kb region of genomic sequence starting with the 5' UTR and including all coding exons as well as part of the 3' UTR were replaced with a floxed neomycin resistance gene. Transient transfection with a cre expressing plasmid then eliminated the neomycin gene. Absence of mRNA transcripts in mice derived from these ES cells was demonstrated by reverse transcription and PCR. (J:85497)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccne2 Mutation: 32 strains or lines available

References

• Original: J:85497 Parisi T, et al., Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells. EMBO J. 2003 Sep 15;22(18):4794-803
• All: 1 reference(s)