Tkt^tm1Jpi
Targeted Allele Detail

Summary

• Symbol: Tkt^tm1Jpi
• Name: transketolase; targeted mutation 1, Joram Piatigorsky
• MGI ID: MGI:2671068
• Synonyms: Tkt -/-
• Gene: Tkt Location: Chr14:30271088-30296681 bp, + strand Genetic Position: Chr14, 18.79 cM, cytoband B1
• Alliance: Tkt^tm1Jpi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78430
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Genomic sequences including intron 2 and exon 3 were replaced with a neomycin selection cassette. Western blot analysis demonstrated that various tissues of heterozygous mice expressed half the amount of encoded protein, and enzymatic assays demonstrated reductions in enzyme activity in various tissues of heterozygous mice. (J:78430)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tkt Mutation: 41 strains or lines available

References

• Original: J:78430 Xu ZP, et al., Transketolase haploinsufficiency reduces adipose tissue and female fertility in mice. Mol Cell Biol. 2002 Sep;22(17):6142-7
• All: 1 reference(s)