Cenpa^tm2Khac
Targeted Allele Detail

Summary

• Symbol: Cenpa^tm2Khac
• Name: centromere protein A; targeted mutation 2, K H Andy Choo
• MGI ID: MGI:2670429
• Synonyms: Cenpa^g
• Gene: Cenpa Location: Chr5:30824214-30832181 bp, + strand Genetic Position: Chr5, 16.76 cM
• Alliance: Cenpa^tm2Khac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84634
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Homologous recombination of a targeting vector containing GFP followed by IRES-neo resulted in the replacement of a genomic fragment extending from the endogenous stop codon in exon 4 through a portion of exon 5. The targed locus was designed to encode a 381 amino acid protein comprised of the endogenous gene followed by an 8 residue linker and GFP. The presence of a protein of the expected size was detected by Western blot analysis of homozygous mutant mice. (J:84634)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpa Mutation: 15 strains or lines available

References

• Original: J:84634 Kalitsis P, et al., Partially functional Cenpa-GFP fusion protein causes increased chromosome missegregation and apoptosis during mouse embryogenesis. Chromosome Res. 2003;11(4):345-57
• All: 1 reference(s)