Dnmt1^tm3Jae
Targeted Allele Detail

Summary

• Symbol: Dnmt1^tm3Jae
• Name: DNA methyltransferase 1; targeted mutation 3, Rudolf Jaenisch
• MGI ID: MGI:2657151
• Synonyms: Dnmt1^chip
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^tm3Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83179
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting vector containing a Dnmt1 cDNA fragment followed by a PGK-puro cassette was inserted upstream of the previously targeted coding region containing a neomycin selection cassette (Dnmt1^tm1Enl) and downstream of the endogenous promoter. Northern blot analysis showed that transcript produced by the inserted cDNA sequence was at a level and size similar to that of the endogenous allele. (J:83179)

Tumor Data

List all tumor models in MMHCdb carrying Dnmt1^tm3Jae

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 108 strains or lines available

Notes

Previously targeted J1 ES cells homozygous for Dnmt1^tm1Enl were electroporated with the targeting vector.

References

• Original: J:83179 Tucker KL, et al., Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev. 1996 Apr 15;10(8):1008-20
• All: 15 reference(s)