Lepr^tm1Mgmj
Targeted Allele Detail

Summary

• Symbol: Lepr^tm1Mgmj
• Name: leptin receptor; targeted mutation 1, Martin G Myers, Jr
• MGI ID: MGI:2653294
• Synonyms: lepr^s, lepr^S1138, s
• Gene: Lepr Location: Chr4:101574601-101672549 bp, + strand Genetic Position: Chr4, 46.96 cM
• Alliance: Lepr^tm1Mgmj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82334
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Tyrosine 1138 of exon 18b was replaced with a serine residue (Y1138S) and a neomycin resistance cassette was inserted into the downstream intron via homologous recombination. The Y1138S amino acid substitution specifically disrupts the leptin receptor long form (LRb)-STAT3 signal. Expression of the knock-in allele in mutant animals was confirmed by semi-quantitative RT-PCR analysis of hypothalamus RNA. (J:82334)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lepr Mutation: 121 strains or lines available

References

• Original: J:82334 Bates SH, et al., STAT3 signalling is required for leptin regulation of energy balance but not reproduction. Nature. 2003 Feb 20;421(6925):856-9
• All: 20 reference(s)