Ppp1r15a^tm1Dron
Targeted Allele Detail

Summary

• Symbol: Ppp1r15a^tm1Dron
• Name: protein phosphatase 1, regulatory subunit 15A; targeted mutation 1, David Ron
• MGI ID: MGI:2652765
• Synonyms: GADD34^deltaC
• Gene: Ppp1r15a Location: Chr7:45172341-45175692 bp, - strand Genetic Position: Chr7, 29.34 cM, cytoband B2
• Alliance: Ppp1r15a^tm1Dron page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82255
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 3 with a PGK-neo cassette via homologous recombination. The targeted mutation deletes residues 549-657 of the PP1c binding domain. Western blot analysis of homozygous mutant fibroblasts detected a truncated protein product. Absence of protein activity was confirmed by immunoprecipitation analysis. (J:82255)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp1r15a Mutation: 23 strains or lines available

References

• Original: J:82255 Novoa I, et al., Stress-induced gene expression requires programmed recovery from translational repression. EMBO J. 2003 Mar 3;22(5):1180-7
• All: 19 reference(s)