Camk2a^tm5Sva
Targeted Allele Detail

Summary

• Symbol: Camk2a^tm5Sva
• Name: calcium/calmodulin-dependent protein kinase II alpha; targeted mutation 5, Alcino Silva
• MGI ID: MGI:2389272
• Synonyms: alphaCaMKII T305D, T305D
• Gene: Camk2a Location: Chr18:61058704-61121224 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Camk2a^tm5Sva page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79714
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Sequence encoding threonine 305 was targeted by homologous recombination of a construct containing an engineered arginine substitution mutation. The replacement of the this threonine residue with the negatively charged argnine was designed to interfere with calcium/calmodulin binding. Western blot analysis confirmed the expression of a normal sized protein. A single loxP site was left within the locus. (J:79714)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Camk2a Mutation: 136 strains or lines available

References

• Original: J:79714 Elgersma Y, et al., Inhibitory Autophosphorylation of CaMKII Controls PSD Association, Plasticity, and Learning. Neuron. 2002 Oct 24;36(3):493-505
• All: 8 reference(s)