Igh^tm3Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm3Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 3, University of Cologne
• MGI ID: MGI:2388492
• Synonyms: D42VDJ, g1D42i, gID42i
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79556
• Parent Cell Line: BL/6-III (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The DQ52 element of Igh-D was disrupted by the insertion of a loxP site flanked neomycin resistance gene cassette and the Igh-J elements were replaced with sequence encoding the variable region of DNA binding antibody D42. In constrast to Igh^tm6Cgn, this allele contains an unmutated Igh-V11 sequence. The Igh intron enhancer (Rr150) was left intact. Flow cytometry verified cell surface expression of the mutant protein product. The floxed neo cassette was removed via cre-mediated recombination. (J:76117)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 45 strains or lines available

References

• Original: J:79556 Sonoda E, et al., B cell development under the condition of allelic inclusion. Immunity. 1997 Mar;6(3):225-33
• All: 9 reference(s)