Igh^tm4Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm4Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 4, University of Cologne
• MGI ID: MGI:2388394
• Synonyms: B1-8f, B1-8H, B1-8+/+Jkappa^-, Igh^B1-8f, VHB1-8, VHB1-8f, VNP
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79299
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Modified regulatory region, No functional change)
• Mutation: Insertion
• Mutation details: The endogenous locus was modified via homologous recombination and subsequent in vitro cre-mediated excision to generate a floxed version of Igh^tm2Cgn. The region flanked by single loxP sites contained the inserted variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8) as well as the Igh intron enhancer (Emu or Rr150). Flow cytometry verified cell surface expression on peripheral B cells obtained from heterozygous and homozygous mice. (J:79299)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 45 strains or lines available

Notes

The targeted IgH allele was of the a allotype.

References

• Original: J:79299 Lam KP, et al., In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death. Cell. 1997 Sep 19;90(6):1073-83
• All: 35 reference(s)