Eif2s1^tm1Rjk
Targeted Allele Detail

Summary

• Symbol: Eif2s1^tm1Rjk
• Name: eukaryotic translation initiation factor 2, subunit 1 alpha; targeted mutation 1, Randal J Kaufman
• MGI ID: MGI:2388264
• Synonyms: eIF2alpha^S51A, eIF2alpha^S/A
• Gene: Eif2s1 Location: Chr12:78908846-78933784 bp, + strand Genetic Position: Chr12, 35.51 cM, cytoband D1
• Alliance: Eif2s1^tm1Rjk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70004
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A serine to alanine substitution at a phosphorylation site at position 51 (S51A) of exon 2 was introduced, and a floxed neomycin resistance cassette was inserted into the upstream intron for positive selection. The floxed neo cassette was removed by cre-mediated recombination leaving behind a single loxP site and the S51A mutation. Western blot analysis of mouse embryonic fibroblasts derived from homozygous mutant animals confirmed expression of the mutant allele. (J:70004)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eif2s1 Mutation: 57 strains or lines available

References

• Original: J:70004 Scheuner D, et al., Translational control is required for the unfolded protein response and in vivo glucose homeostasis. Mol Cell. 2001 Jun;7(6):1165-76
• All: 37 reference(s)