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Targeted Allele Detail
Symbol: Slc22a3tm1Dpb
Name: solute carrier family 22 (organic cation transporter), member 3; targeted mutation 1, Denise P Barlow
MGI ID: MGI:2388117
Synonyms: Oct3-, Orct3-deficient
Gene: Slc22a3  Location: Chr17:12638859-12726591 bp, - strand  Genetic Position: Chr17, 8.52 cM
Alliance: Slc22a3tm1Dpb page
Germline Transmission:  Earliest citation of germline transmission: J:69978
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExon 1 and flanking intronic sequences were deleted and replaced with a floxed PGK-neo cassette via homologous recombination. Transient expression of cre recombinase in correctly targeted cells removed the selection cassette leaving behind a single loxP site in place of exon 1. Gene expression was absent in the heart, brain, and skeletal muscle of homozygous mutant animals as determined by Northern blot analysis. However, an aberrant transcript was detected in placentas from homozygous mutant embryos. RT-PCR analysis revealed the mutant transcript results from alternative splicing of intron 2 to exon 3 and does not translate a functional protein product. (J:69978)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Slc22a3 Mutation:  29 strains or lines available
Original:  J:69978 Zwart R, et al., Impaired activity of the extraneuronal monoamine transporter system known as uptake-2 in Orct3/Slc22a3-deficient mice. Mol Cell Biol. 2001 Jul;21(13):4188-96
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.24
The Jackson Laboratory