Nid2^{Gt(GST011)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Nid2^{Gt(GST011)Byg}
• Name: nidogen 2; gene trap GST011, BayGenomics
• MGI ID: MGI:2388069
• Synonyms: Nid2^-, Nid2^{GSF(pGT1.8TM)011Ska}, Nid2^{Gt(pGT1.8TM)11Wcs}, nidogen-2^-
• Gene: Nid2 Location: Chr14:19801333-19861855 bp, + strand Genetic Position: Chr14, 10.73 cM
• Alliance: Nid2^{Gt(GST011)Byg} page

Mutation origin

• Mutant Cell Line: GST011
• Germline Transmission: Earliest citation of germline transmission: J:78947
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1.8TM
• Mutation details: The gene was disrupted by the random integration of the pGT1.8TM gene trap vector into intron 4, 414 bp downstream of the 3' end of exon 4. While normal transcript was undetected, fusion transcript consisting of endogenous (exons 1 through 4) and vector sequence was detected by Northern blot analysis. Western blot analyses followed by radioimmunoassays showed a near complete ablation of protein expression (less than 1%). (J:78947)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  GST011	CC144826 ; (MGI Seq Detail)	5' RACE	Chr14:19818698-19819003 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  25 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nid2 Mutation: 56 strains or lines available

References

• Original: J:78947 Schymeinsky J, et al., Gene structure and functional analysis of the mouse nidogen-2 gene: nidogen-2 is not essential for basement membrane formation in mice. Mol Cell Biol. 2002 Oct;22(19):6820-30
• All: 10 reference(s)