Trpc4^tm1Bni
Targeted Allele Detail

Summary

• Symbol: Trpc4^tm1Bni
• Name: transient receptor potential cation channel, subfamily C, member 4; targeted mutation 1, Bernd Nilius
• MGI ID: MGI:2387666
• Synonyms: TRP4^-/-
• Gene: Trpc4 Location: Chr3:54063456-54225892 bp, + strand Genetic Position: Chr3, 25.43 cM, cytoband D
• Alliance: Trpc4^tm1Bni page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68178
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 278 bp of the exon encoding transmembrane domains 4 and 5 and the adjacent downstream splice donor site were replaced with a PGK-neo cassette in the reverse transcriptional orientation. Northern blot and Western blot of brain homogenates and primary mouse vascular endothelial cells (MAECs) cultured from homozygous mutant animals confirmed the absence of gene expression. (J:68178)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trpc4 Mutation: 62 strains or lines available

References

• Original: J:68178 Freichel M, et al., Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice. Nat Cell Biol. 2001 Feb;3(2):121-7
• All: 24 reference(s)