Ret^tm2(RET)Vpa
Targeted Allele Detail

Summary

• Symbol: Ret^tm2(RET)Vpa
• Name: ret proto-oncogene; targeted mutation 2, Vassilis Pachnis
• MGI ID: MGI:2387446
• Synonyms: miRet⁵¹, Ret⁵¹
• Gene: Ret Location: Chr6:118128706-118174679 bp, - strand Genetic Position: Chr6, 55.86 cM, cytoband E3-F1
• Alliance: Ret^tm2(RET)Vpa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71588
• Parent Cell Line: E14.2 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Ret^tm2(RET)Vpa expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RET (5979)

  Ret	(MGI:97902)

  expresses the RET51 isoform

• Mutation details: A cDNA fragment encoding the intracelluar portion of the human RET51 isoform was fused in-frame with mouse exon 11 immediately downstream of the transmembrane domain-coding region. A floxed PGK-neo cassette followed the human cDNA sequence for positive selection. RT-PCR analysis and Western blot using allele-specific primers and antibodies, respectively, confirmed expression of the chimeric gene product in homozygous mutant animals. Semiquanititative RT-PCR and Western blot also determined the expression level of the mutant allele was similar to that of wild-type. (J:71588)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ret Mutation: 53 strains or lines available

References

• Original: J:71588 de Graaff E, et al., Differential activities of the RET tyrosine kinase receptor isoforms during mammalian embryogenesis. Genes Dev. 2001 Sep 15;15(18):2433-44
• All: 13 reference(s)