Cckbr^tm1Tom
Targeted Allele Detail

Summary

• Symbol: Cckbr^tm1Tom
• Name: cholecystokinin B receptor; targeted mutation 1, Toshimitsu Matsui
• MGI ID: MGI:2384535
• Synonyms: CCK2^-, CCK-2R^-, CCK2R^-, CCK2RKO, CCKBR^-
• Gene: Cckbr Location: Chr7:105075201-105085546 bp, + strand Genetic Position: Chr7, 55.86 cM
• Alliance: Cckbr^tm1Tom page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35876
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 2 and exons 3-5 were replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the majority of 7 transmembrane regions after amino acid 108. Absence of gene expression was demonstrated by Northern blot analysis of cerebral cortex, basal ganglion, stomach, and pancreas mRNA from homozygous mutant animals using a 2.3 kb cDNA fragment as the probe. Absence of functional receptor protein was confirmed via binding assays using brain cell membrane preparations from homozygous mutants. (J:35876)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cckbr Mutation: 23 strains or lines available

References

• Original: J:35876 Nagata A, et al., G protein-coupled cholecystokinin-B/gastrin receptors are responsible for physiological cell growth of the stomach mucosa in vivo. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11825-30
• All: 35 reference(s)