Il4^tm1Rao
Targeted Allele Detail

Summary

• Symbol: Il4^tm1Rao
• Name: interleukin 4; targeted mutation 1, Anjana Rao
• MGI ID: MGI:2384290
• Synonyms: Rr168^tm1Rao, V/Va KO
• Gene: Il4 Location: Chr11:53503287-53509492 bp, - strand Genetic Position: Chr11, 31.97 cM
• Alliance: Il4^tm1Rao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78089
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: 3.7 kb of sequence in the intergenic region between Il4 and Kif3a was replaced with a loxP site flanked neomycin resistance gene cassette. The deleted region contains DNAse I hypersensitivity sites V and Va. These sites contain enhancers for Il4 transcription. The neo cassette was excised in vitro via the expression of cre recombinase. T lymphocytes and mast cells from homozygous mutant mice express severely reduced Il4 mRNA and protein. (J:78089)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il4 Mutation: 42 strains or lines available

References

• Original: J:78089 Solymar DC, et al., A 3' enhancer in the IL-4 gene regulates cytokine production by Th2 cells and mast cells. Immunity. 2002 Jul;17(1):41-50
• All: 2 reference(s)