Fos^{tm2(Fosl1)Wag}
Targeted Allele Detail

Summary

• Symbol: Fos^{tm2(Fosl1)Wag}
• Name: FBJ osteosarcoma oncogene; targeted mutation 2, Erwin F Wagner
• MGI ID: MGI:2384074
• Synonyms: Fos^Fosl1, Fos^Fra1
• Gene: Fos Location: Chr12:85520664-85524047 bp, + strand Genetic Position: Chr12, 39.7 cM
• Alliance: Fos^{tm2(Fosl1)Wag} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65766
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Fos^{tm2(Fosl1)Wag} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Fosl1

• Mutation details: Exon 1 of the Fos locus was replaced with the entire Fosl1 coding sequence followed by stop codons. A floxed neo cassette was inserted for selection and subsequently excised from ES cells via cre mediated recombination. Because Fos mRNA levels are in part controlled by a destabilizing region in the 3' UTR, Fos sequence downstream of the Fosl1 insertion was left intact, though affected by a frameshift mutation introduced by the insertion. The lack of aberrant transcripts was verified by RT-PCR analysis. RNAse protection assays confirmed that the expression pattern of the inserted Fosl1 recapitulated that of the endogenous Fos gene. (J:65766)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fos Mutation: 42 strains or lines available

References

• Original: J:65766 Fleischmann A, et al., Fra-1 replaces c-fos-dependent functions in mice. Genes Dev. 2000 Nov 1;14(21):2695-700
• All: 4 reference(s)