Cd55^tm1Mme
Targeted Allele Detail

Summary

• Symbol: Cd55^tm1Mme
• Name: CD55 molecule, decay accelerating factor for complement; targeted mutation 1, M Edward Medof
• MGI ID: MGI:2383980
• Synonyms: Cd55^-, Daf1^-
• Gene: Cd55 Location: Chr1:130366764-130390481 bp, - strand Genetic Position: Chr1, 56.89 cM
• Alliance: Cd55^tm1Mme page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72210
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was introduced with a neo-TK cassette into exon 3 and a second loxP site was introduced with a TK-Hph cassette into exon 5 via homologous recombination. Transient expression of cre recombinase in correctly targeted ES cells removed floxed regions containing exons 3-5 and the 2 TK genes. The deleted exons encode complement control protein (CCP) modules 2 and 3. RT-PCR analysis detected a truncated mRNA in homozygous mutant animals lacking exons 3-5. Functional assays confirmed the mutant protein was devoid of protein activity. (J:72210)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd55 Mutation: 40 strains or lines available

References

• Original: J:72210 Lin F, et al., Tissue distribution of products of the mouse decay-accelerating factor (DAF) genes. Exploitation of a Daf1 knock-out mouse and site-specific monoclonal antibodies. Immunology. 2001 Oct;104(2):215-25
• All: 30 reference(s)