Lat^tm2.1Lov
Targeted Allele Detail

Summary

• Symbol: Lat^tm2.1Lov
• Name: linker for activation of T cells; targeted mutation 2.1, Paul E Love
• MGI ID: MGI:2183741
• Synonyms: LAT^4YF
• Gene: Lat Location: Chr7:125962996-125968742 bp, - strand Genetic Position: Chr7, 69.01 cM
• Alliance: Lat^tm2.1Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77695
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: This allele was generated by breeding mice carrying Lat^tm2Lov with transgenic mice expressing cre recombinase via the EIIa promoter. Expression of cre resulted in the excision of the floxed neo cassette, leaving the tyrosine to phenylalanine mutations at positions identified as being critical for the binding of signaling molecules (136, 175, 195, and 235) intact. Flow cytometric analysis of thymocytes from homozygous mutants showed reduced levels of protein. (J:77695)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lat Mutation: 9 strains or lines available

References

• Original: J:77695 Sommers CL, et al., Knock-in mutation of the distal four tyrosines of linker for activation of T cells blocks murine T cell development. J Exp Med. 2001 Jul 16;194(2):135-42
• All: 3 reference(s)