Mbd4^tm1Bird
Targeted Allele Detail

Summary

• Symbol: Mbd4^tm1Bird
• Name: methyl-CpG binding domain protein 4; targeted mutation 1, Adrian Bird
• MGI ID: MGI:2183491
• Synonyms: Mbd4^-
• Gene: Mbd4 Location: Chr6:115817658-115830332 bp, - strand Genetic Position: Chr6, 53.72 cM
• Alliance: Mbd4^tm1Bird page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77896
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A cassette, containing a splice acceptor site and IRES-Beta-geo, was inserted into intron 1 of the endogenous locus. Northern blot and RT-PCR analysis showed levels of transcript in homozygous mutant mice to be less than 0.1% of those in wild-type mice. Two truncated transcripts were identified; one which truncated at the transgenic polyadenylation signal and another in which a frameshift mutation was introduced by the excision of the IRES-Beta-geo transgenic region (leaving the the splice acceptor site in between exons 1 and 2). (J:77896)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbd4 Mutation: 31 strains or lines available

References

• Original: J:77896 Millar CB, et al., Enhanced CpG mutability and tumorigenesis in MBD4-deficient mice. Science. 2002 Jul 19;297(5580):403-5
• All: 3 reference(s)