Gata2^tm2Sho
Targeted Allele Detail

Summary

• Symbol: Gata2^tm2Sho
• Name: GATA binding protein 2; targeted mutation 2, Stuart Orkin
• MGI ID: MGI:2183390
• Synonyms: GATA-2^KI, GATA-2^V296G
• Gene: Gata2 Location: Chr6:88170873-88184014 bp, + strand Genetic Position: Chr6, 39.2 cM
• Alliance: Gata2^tm2Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77724
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A valine to glycine substitute at amino acid 296 (V296G) of exon 4, a floxed neo cassette insertion in the downstream intron, and a loxP site upstream of exon 4, were introduced to the gene via homologous recombination. The V296G mutation disrupts the FOG-binding region without affecting DNA-binding ability. The neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. (J:77724)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata2 Mutation: 26 strains or lines available

References

• Original: J:77724 Chang AN, et al., GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9237-42
• All: 2 reference(s)