Cebpa^{tm1.1(Cebpb)Ylee}
Targeted Allele Detail

Summary

• Symbol: Cebpa^{tm1.1(Cebpb)Ylee}
• Name: CCAAT/enhancer binding protein alpha; targeted mutation 1.1, Ying-Hue Lee
• MGI ID: MGI:2183333
• Synonyms: C/ebpa-alpha^beta
• Gene: Cebpa Location: Chr7:34818718-34821353 bp, + strand Genetic Position: Chr7, 21.02 cM
• Alliance: Cebpa^{tm1.1(Cebpb)Ylee} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77646
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Cebpa^{tm1.1(Cebpb)Ylee} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cebpb (MGI:88373)

• Mutation details: This allele was generated by breeding mice homozygous for Cebpa^{tm1(Cebpb)Ylee} with transgenic mice expressing cre recombinase, driven by the EIIa promoter. Expression of cre resulted in the excision of the floxed neo gene downstream of the stop codon. The Cebpb coding region inserted at the endogenous start codon to replace the coding region of Cebpa remained intact. Northern blot analysis confirmed the absence of Cebpa mRNA and the presence of the Cebpa/Cebpb hybrid mRNA in homozygous mutant mice. Western blot analysis showed that Cebpb protein levels were concomitant with the combined amount of Cebpb and Cebpa/Cebpb transcript until the age of 7 weeks. (J:77646)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cebpa Mutation: 16 strains or lines available

References

• Original: J:77646 Chen SS, et al., C/EBPbeta, when expressed from the C/ebpalpha gene locus, can functionally replace C/EBPalpha in liver but not in adipose tissue. Mol Cell Biol. 2000 Oct;20(19):7292-9
• All: 5 reference(s)