Ucp3^tm1Lowl
Targeted Allele Detail

Summary

• Symbol: Ucp3^tm1Lowl
• Name: uncoupling protein 3 (mitochondrial, proton carrier); targeted mutation 1, Bradford B Lowell
• MGI ID: MGI:2183166
• Gene: Ucp3 Location: Chr7:100122198-100135639 bp, + strand Genetic Position: Chr7, 54.36 cM
• Alliance: Ucp3^tm1Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62313
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 700 bp genomic fragment containing part of exons 2 and 3 was replaced with a neomycin selection cassette. RNase protection analysis on RNA derived from skeletal muscle and brown adipose tissue of homozygous mice confirmed that no detectable transcript was expressed from this allele. Western blot analysis confirmed the absence of detectable protein in skeletal muscle mitochondria of homozygous mice. (J:62313)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ucp3 Mutation: 4 strains or lines available

References

• Original: J:62313 Vidal-Puig AJ, et al., Energy metabolism in uncoupling protein 3 gene knockout mice. J Biol Chem. 2000 May 26;275(21):16258-66
• All: 8 reference(s)