Ppef1^tm1Nat
Targeted Allele Detail

Summary

• Symbol: Ppef1^tm1Nat
• Name: protein phosphatase with EF hand calcium-binding domain 1; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:2182810
• Synonyms: PPEF-1^-
• Gene: Ppef1 Location: ChrX:159406090-159518761 bp, - strand Genetic Position: ChrX, 73.95 cM
• Alliance: Ppef1^tm1Nat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72941
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The last 21 codons of exon 5 were replaced with a lacZ-neo cassette via homologous recombination and results in an in-frame fusion of the lacZ gene to exon 5. Gene expression was absent in retinas and brain stem of homozygous mutant animals as determined by RT-PCR analysis. Beta-galactosidase activity was not present in the mutant animals. (J:72941)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppef1 Mutation: 3 strains or lines available

References

• Original: J:72941 Ramulu P, et al., Normal Light Response, Photoreceptor Integrity, and Rhodopsin Dephosphorylation in Mice Lacking Both Protein Phosphatases with EF Hands (PPEF-1 and PPEF-2). Mol Cell Biol. 2001 Dec;21(24):8605-14
• All: 1 reference(s)