Efnb2^tm3.1Kln
Targeted Allele Detail

Summary

• Symbol: Efnb2^tm3.1Kln
• Name: ephrin B2; targeted mutation 3.1, Rudiger Klein
• MGI ID: MGI:2182632
• Synonyms: ephrinB2^deltaC
• Gene: Efnb2 Location: Chr8:8667434-8711242 bp, - strand Genetic Position: Chr8, 3.42 cM
• Alliance: Efnb2^tm3.1Kln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67296
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Mice heterozygous for Efnb2^tm3Kln were bred with mice expressing a cre recombinase transgene, resulting in the excision of the floxed neo gene. The inserted cDNA encoding the truncated protein lacking 66 residues of carboxy terminal region and containing a hemagglutinin (HA) epitope tag, was left intact. The expression pattern of the targeted allele was determined to resemble that of the wild-type allele via in situ hybridization. Analysis using modified receptors showed localization to the cell surface. Production of protein with a reduced molecular weight was verified by immunoblotting with an anti-HA antibody. (J:67296)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efnb2 Mutation: 28 strains or lines available

References

• Original: J:67296 Adams RH, et al., The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration. Cell. 2001 Jan 12;104(1):57-69
• All: 1 reference(s)