Ung^tm1Tld
Targeted Allele Detail

Summary

• Symbol: Ung^tm1Tld
• Name: uracil DNA glycosylase; targeted mutation 1, Tomas Lindahl
• MGI ID: MGI:2182610
• Synonyms: ung^-
• Gene: Ung Location: Chr5:114268447-114277384 bp, + strand Genetic Position: Chr5, 55.97 cM, cytoband F
• Alliance: Ung^tm1Tld page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63056
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a neomycin selection cassette disrupted exon 4, which encodes residues of the uracil-binding pocket. The insertion was designed to impair the production of both nuclear and mitochondrial isoforms. Uracil-DNA glycosylase activity was shown to be markedly reduced in cell extracts from homozygous mutant mice. The residual activity was not inhibited by antibodies raised against the encoded protein. (J:63056)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ung^tm1Tld

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ung Mutation: 23 strains or lines available

References

• Original: J:63056 Nilsen H, et al., Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication. Mol Cell. 2000 Jun;5(6):1059-65
• All: 43 reference(s)