Gast^tm1Lcs
Targeted Allele Detail

Summary

• Symbol: Gast^tm1Lcs
• Name: gastrin; targeted mutation 1, Linda C Samuelson
• MGI ID: MGI:2182563
• Synonyms: G-, GasKO, Gast^o
• Gene: Gast Location: Chr11:100225233-100227822 bp, + strand Genetic Position: Chr11, 63.46 cM
• Alliance: Gast^tm1Lcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77780
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: All 3 exons of the gene were replaced with a neomycin resistance gene via homologous recombination. Complete absence of gene transcripts in stomachs of homozygous mutant animals was verified by Northern blot analysis. (J:77780, J:102142)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gast Mutation: 18 strains or lines available

References

• Original: J:77780 Friis-Hansen L, et al., Impaired gastric acid secretion in gastrin-deficient mice. Am J Physiol. 1998 Mar;274(3 Pt 1):G561-8
• All: 28 reference(s)