Pim1^tm1Pwl
Targeted Allele Detail

Summary

• Symbol: Pim1^tm1Pwl
• Name: Pim1, proto-oncogene, serine/threonine kinase; targeted mutation 1, Peter W Laird
• MGI ID: MGI:2182396
• Synonyms: Pim1-, Pim1neo59
• Gene: Pim1 Location: Chr17:29709759-29715085 bp, + strand Genetic Position: Chr17, 15.38 cM
• Alliance: Pim1^tm1Pwl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:15268
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced the promoter region, both transcriptional and translational start sites, and a portion of the coding sequence containing an essential lysine residue in the ATP-binding site of the protein kinase domain. An in vitro autophosphorylation assay showed an absence of phospho-kinase activity in splenocytes obtained from homozygous mutant mice. (J:15268)

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pim1 Mutation: 14 strains or lines available

References

• Original: J:15268 Laird PW, et al., In vivo analysis of Pim-1 deficiency. Nucleic Acids Res. 1993 Oct 11;21(20):4750-5
• All: 30 reference(s)